Block staining with p-phenylenediamine for light microscope autoradiography.

Autoradiography with the Electron Microscope

Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...

Oxidized p-phenylenediamine staining of epoxy resin sections.

Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable ele...

Corneal endothelial autoradiography with the scanning electron microscope.

A new technique for performing corneal endothelial cell autoradiography with the scanning electron microscope is presented. The scanning electron microscope is able to detect silver grains deposited over tritiated thymidine--labeled nuclei of regenerating corneal endothelium.

A Method for Intracellular Autoradiography in the Electron Microscope

A technic is described for high resolution intracellular autoradiography in the electron microscope. Cultures of LLC-MK(2) monkey kidney cells were incubated for 72 hours in a medium containing 0.4 microcurie per ml of thymidine-H(3). After labeling, the cells were fixed with osmium tetroxide and embedded in methacrylate. Ultrathin sections of the labeled tissue were taken up on Formvar-coated ...

University of Groningen P - phenylenediamine